Urolithin A
CAS 1143-70-0 — Research-Grade Mitophagy Inducer

Direct-from-manufacturer supply of synthetic Urolithin A for preclinical and analytical laboratories: 99.5% HPLC purity, batch-level chromatographic data published on this page, and a packing ladder that runs from a 100 mg evaluation pack to 10 kg commercial batches.

QC Tested — HPLC + LC-MS + NMR In Stock Samples Available Global Delivery Batch COA Included Custom Synthesis 100 mg – 10 kg For Research Use Only
Request Quotation View Batch Data
99.5%
HPLC Purity (batch label)
6
Pack Sizes, 100 mg – 10 kg
229.0
m/z [M+H]+ Confirmed by LC-MS

Compound Identity

Name: Urolithin A
CAS: 1143-70-0
Formula: C13H8O4
MW: 228.20 g/mol
IUPAC: 3,8-dihydroxy-6H-dibenzo[b,d]pyran-6-one
Class: Dibenzopyranone (urolithin)
Precursor: Ellagitannins (pomegranate, walnut)
InChIKey: RIUPLDUFZCXCHM-UHFFFAOYSA-N
PubChem CID: 5488186
Appearance: Off-white to pale yellow powder
Purity: ≥98% spec; 99.5% by HPLC (current batch)
Storage: −20 °C, protected from light and moisture
Use: For Research Use Only
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CAS Number
1143-70-0
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Purity (HPLC)
≥ 98% · 99.5% batch
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Molecular Weight
228.20 g/mol
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Stock Status
Research packs in stock

Batch Analytical Data: HPLC, LC-MS and 1H NMR

Every quotation from NutraBiotechChem is backed by batch-specific instrumental data. The full data package behind the current Urolithin A batch is reproduced below — the same spectra and chromatograms ship as PDFs with each order.

1H NMR · 400 MHz · DMSO-d6Identity
1H NMR spectrum of Urolithin A at 400 MHz in DMSO-d6 showing aromatic and phenolic proton signals between 10.40 and 7.32 ppm
Structural identity by proton NMR. Seven resolved signals between 10.40 and 7.32 ppm match the 3,8-dihydroxy-6H-dibenzo[b,d]pyran-6-one framework: two phenolic OH protons downfield and five aromatic protons with the expected coupling pattern. Integral ratios read 1.36 : 1.00 : 1.04 : 1.00 : 1.02 : 0.99 : 1.06 against the internal reference, consistent with the molecular formula C13H8O4. Residual DMSO-d5 appears at 2.504 ppm.Bruker 400 MHz · SF 400.1300000 MHz · DMSO-d6 · acquisition 2021-06-08
HPLC-DAD · 214 / 254 nmPurity
HPLC-DAD chromatogram of Urolithin A at 214 nm and 254 nm with a single peak at 6.796 minutes integrating to 100 area percent
Chromatographic purity. A single symmetry peak at RT 6.796 min integrates to 100.00 area% at both detection wavelengths, with peak height 1457.41 mAU and peak width 0.29 min. No secondary peaks above baseline noise are observed across the 20–80% gradient run.Agilent HPLC · method 20-80_20min_HPLC.amx · sample A-5213 · 2021-06-15
LC-MS · DAD + TIC · ESI(+)Identity
LC-MS chromatograms of Urolithin A showing DAD traces at 254 and 214 nm and the total ion current with a single peak at 3.48 minutes
LC-MS inlet chromatograms. DAD traces at 254.4 nm and 214.4 nm each show a single peak (RT 3.48 min / 3.49 min, 100.00 area% at both wavelengths), and the MSD total ion current shows one component at 3.63 min. Injection volume 2 µL, fragmentor 70 V, positive-mode API.OpenLab Chem32 · sample C-197 · injected 2021-06-28
LC-MS · Positive SpectrumIdentity
Positive-mode electrospray mass spectrum of Urolithin A with base peak at m/z 229.0 and fragment ion at m/z 201.0
Molecular identity by MS. The positive-spectrum base peak at m/z 229.0 corresponds to [M+H]+ (calculated 229.05 for C13H9O4+), with a characteristic decarbonylation fragment at m/z 201.0 (−28 Da, loss of CO) and a minor adduct at m/z 249.0. No m/z 303 signal (ellagic acid) is detected, confirming the absence of precursor carryover.ES-API positive · RT 3.63 min · scan 100–1200 m/z
ParameterResultMethod / Comment
AppearanceOff-white to pale yellow powderVisual, against reference
Identity — MSm/z 229.0 [M+H]+; 201.0 (loss of CO)LC-MS, ESI positive
Identity — NMR7 signals, 10.40–7.32 ppm, integral ratios consistent1H NMR, 400 MHz, DMSO-d6
Purity @ 214 nm100.00 area% (single peak)HPLC-DAD
Purity @ 254 nm100.00 area% (single peak)HPLC-DAD
Purity on batch label99.5%Label specification, batch 500 g pack
Impurity screenNo ellagic acid (m/z 303) detectedLC-MS, precursor carryover check
RT consistencyHPLC 6.796 min · LC-MS TIC 3.63 minDifferent methods — both single-peak

Report sample references: HPLC sample A-5213 (2021-06-15), LC-MS sample C-197 (2021-06-28), NMR acquisition 2021-06-08. Reports from different instruments in the same release workflow carry different sample codes; the PDF package shipped with an order pairs all documents to the same batch number. A fuller specification breakdown is maintained on the Urolithin A product page.

Urolithin A from the Manufacturer

What research-grade Urolithin A actually is, how it is produced, and why the analytical package matters as much as the purity number.

Urolithin A (CAS 1143-70-0) is a dibenzopyranone metabolite produced in nature by gut microorganisms from ellagitannins and ellagic acid found in pomegranate, walnut and berry sources. Because the metabotype that converts these precursors into urolithin A is present in only a fraction of the population, essentially all laboratory work uses synthetic Urolithin A that is structurally identical to the microbial metabolite — which is exactly what NutraBiotechChem supplies.

The compound is the most widely cited small-molecule inducer of mitophagy, the selective autophagic clearance of damaged mitochondria, and has become a standard tool compound in mitochondrial quality-control, bioenergetics and gut-microbiota metabolite pharmacology research. Publication volume on urolithin A has grown steadily since the first mitophagy characterization studies, which keeps demand high across both milligram research packs and multi-kilogram screening programs.

For procurement teams, two practical realities drive sourcing decisions. First, "99% Urolithin A" is not a specification until the method is stated — HPLC wavelength, gradient and integration basis all change the number. Second, the difference between an importable, experiment-ready batch and a failed one is usually the documentation package, not the price. Both points are addressed head-on in the pricing and documentation sections below.

NutraBiotechChem supplies Urolithin A across a six-step packing ladder (100 mg to 10 kg), supports custom purity targets and custom synthesis of urolithin analogs, and ships every pack with its batch certificate of analysis. The material is supplied strictly for research use only and is not offered for human or veterinary use.

Urolithin A CAS 1143-70-0 powder, 500 g net weight, in a sealed aluminium pouch with batch label showing 99.5% purity
Typical pack: sealed moisture-barrier pouch, batch label showing compound name, CAS 1143-70-0, purity (99.5% for the pictured batch) and net weight. Smaller packs (100 mg – 10 g) are supplied in amber glass vials with PTFE-lined caps.

Why Laboratories Buy Urolithin A

The research logic behind demand — from precursor metabolism to the readouts that end up in study protocols.

1

A gut-microbiota metabolite

Ellagitannins are converted by gut bacteria through urolithin M5 to urolithin A; individuals fall into metabotypes producing urolithin A, B or neither (0). Synthetic supply removes this biological variability from experiments.

2

Mitophagy induction

Urolithin A induces selective mitophagy and promotes autophagosome–lysosome flux (LC3-I to LC3-II conversion) in cellular models, without acting as a protonophoric uncoupler. This distinguishes it from CCCP/FCCP-type controls.

3

Downstream readouts

Published cellular readouts include mitochondrial respiration metrics, mtROS, mitochondrial membrane potential markers, AMPK and PI3K–Akt–mTOR pathway modulation, and AhR binding — the assays most procurement requests are built around.

Mitophagy Autophagic flux LC3-I / LC3-II PINK1 / Parkin pathway AMPK PI3K–Akt–mTOR AhR Mitochondrial membrane potential mtDNA copy number Nrf2 / KEAP1 NLRP3 inflammasome Urolithin metabotypes

Purity Grades & Pricing Logic

There is no single "price of Urolithin A". The quotation is an output of the specification — here is exactly how it is built.

GradeSpecificationTypical UseLead Time
≥98% (HPLC)Catalog grade, method stated on COAIn-vitro mechanism studies, formulation feasibility, exploratory workIn stock
≥99% (HPLC)Extra purification step, batch-quotedQuantitative reference work, method validation, stability studiesMade to order

💰 Seven Variables Behind Every Quote

  • Quantity100 mg → 10 kg
  • Purity grade≥98% or ≥99%
  • Analytical scopeHPLC / MS / NMR / residual solvents
  • Document packageCOA, chromatograms, TDS
  • Packaging formatVial vs aluminium pouch
  • Delivery termsEXW / FOB / CIF / DDP
  • Payment & lead timeCase by case

📈 Pack Ladder & Unit-Cost Logic

  • 100 mg — evaluationIn stock
  • 1 g — study scaleIn stock
  • 10 g — screening roundsIn stock
  • 100 g — pilot scaleMade to order
  • 1 kg — commercialMade to order
  • 10 kg — industrialMade to order

Per-gram cost is highest at 100 mg and can be an order of magnitude lower at 10 kg, because weighing, filling and per-batch HPLC release are fixed costs. The curve flattens noticeably between 10 g and 100 g. A full buyer's guide — including total-cost-of-ownership items such as freight, duties and arrival re-testing — is published in the Urolithin A price analysis article.

Research & Procurement Applications

Where Urolithin A from NutraBiotechChem is routinely specified by purchasing teams.

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Mitophagy & Autophagy Research

Standard tool compound for mitophagy induction and autophagosome–lysosome flux assays, alongside classical controls.

Cell biology
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Mitochondrial QC & Bioenergetics

Used in respiration assays, mtROS and mitochondrial membrane-potential measurement panels in academia and CROs.

Bioenergetics
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Gut-Metabolite Pharmacology

Model microbial metabolite for studies on the colonized-gut chemistry axis; pairs naturally with urolithin B sourcing programs.

Microbiota
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Analytical & Reference Work

≥99% material for method development, system suitability and quantitative reference standards.

Analytical
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Inflammasome & Signaling Studies

Applied in NLRP3 inflammasome, NF-κB-linked and Nrf2/KEAP1 oxidative-stress readout panels.

Signal transduction
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Formulation & Scale-Up Development

Solubility, stability and excipient-compatibility screening, with 100 g – 10 kg supply for downstream programs.

Process development

Comparing urolithins? See the Urolithin A vs Urolithin B comparison and the Urolithin B 99% HPLC powder profile. For a mitochondrial-targeted peptide tool compound, NutraBiotechChem also supplies Elamipretide (SS-31).

Key Literature

Representative publications that reference urolithin A as a research compound.

Ryu D, et al. Nat Med. 2016;22(8):879–888 — first characterization of urolithin A as an inducer of mitophagy in model organisms.
doi:10.1038/nm.4132
Andreux PA, et al. Nat Metab. 2019;1(6):595–603 — human pilot study with urolithin A oral intake in healthy elderly subjects.
doi:10.1038/s42255-019-0073-4
Cerdá B, et al. J Agric Food Chem. 2005;53(14):5571–5576 — identification of urolithin A as a human microbial metabolite of ellagic acid.
doi:10.1021/jf050204n
Espín JC, et al. Evid Based Complement Alternat Med. 2013;2013:270418 — review of ellagitannin metabolism and urolithin metabotype distribution.
doi:10.1155/2013/270418
Singh K, et al. Nat Commun. 2019;10:89 — report on urolithin A effects in a C. elegans aging-related model.
doi:10.1038/s41467-018-07825-5
Method references — routine QC methods used for urolithin A release testing.
HPLC-DAD 20–80% gradient / ESI-MS positive mode / 1H NMR 400 MHz DMSO-d6

Quality, Documentation & Shipping

The paperwork that decides whether a batch is usable on arrival — specified up front, not discovered at customs.

✅ Quality Control

  • IdentityLC-MS + NMR
  • PurityHPLC-DAD, 2 wavelengths
  • Impurity screenPrecursor carryover
  • AppearanceAgainst reference
  • Batch traceabilityEvery pack numbered

📄 Documentation

  • COABatch-specific PDF
  • ChromatogramsHPLC, LC-MS included
  • TDSOn request
  • Origin / customsExport doc set
  • Re-test supportArrival QC data

🚚 Shipping & Logistics

  • Stock packsShips on schedule
  • Custom batchesPer production plan
  • IncotermsEXW / FOB / CIF / DDP
  • Small packsAir freight preferred
  • ClassificationNon-hazardous solid

Detailed logistics terms, packaging-in-transit and destination-storage guidance are on the shipping page. GMP-adjacent programs should budget for independent arrival re-testing — we release the instrument data needed to make that re-test meaningful. Every pack is labelled For Research Use Only and ships with its batch COA.

Frequently Asked Questions

Procurement-side questions we answer by email every week — collected here so the answers are available before the first inquiry.

Which purity grade should I order, ≥98% or ≥99%?
For cell-culture mechanism work and screening, the catalog ≥98% grade is sufficient and is the standard choice. Step up to ≥99% when the compound is used as a quantitative reference, for method validation, or in stability studies, because the extra purification step reduces the minor-peak envelope visible at high loading. Note that a purity claim is only meaningful together with its method — our COA states the HPLC gradient, detection wavelengths and integration basis, and the current batch measures 99.5%.
What pack sizes are available, and where do unit prices move most?
The ladder runs 100 mg, 1 g, 10 g, 100 g, 1 kg and 10 kg. Unit cost per gram is highest at 100 mg, because weighing, filling and per-batch HPLC release are fixed costs spread over the smallest quantity, and it can be roughly an order of magnitude lower at the 10 kg industrial tier. The curve flattens noticeably between 10 g and 100 g, which is why pilot programs often consolidate at 100 g rather than ordering repeated 10 g units.
Can I get a sample before committing to a bulk order?
Yes. A 100 mg research pack serves as the evaluation sample for most programs and normally ships from stock; it carries the same batch COA and chromatographic data as any larger pack, so what you qualify is what you later receive. For kilogram-scale tenders we can arrange a dedicated sample batch and align its analytical scope with your incoming-inspection requirements.
What documentation ships with each batch?
Every pack ships with a batch-specific certificate of analysis covering identity (LC-MS molecular ion and NMR data), HPLC purity at 214 and 254 nm, appearance and storage conditions, and it references the same batch number printed on the label. HPLC chromatograms and LC-MS spectra are included as PDFs, and a technical data sheet plus export documentation set are available for customs handling. The full data package behind the current batch is published in the analytical section of this page.
What are typical lead times for in-stock and made-to-order packs?
Research packs of 100 mg to 10 g are generally in stock and dispatch on the normal shipping schedule after payment confirmation. The 100 g to 10 kg tiers and the ≥99% grade are produced to order, with lead time depending on the production queue at the time of inquiry — the additional purification step for the 99% grade adds measurable time and is quoted case by case. Ask for the current schedule together with your quotation request.
Which delivery terms do you support?
Standard incoterms — EXW, FOB, CIF and DDP — are supported, and we can quote two or more terms side by side so the landed-cost comparison is visible before you commit. Small packs move best by air freight to minimize moisture exposure and transit time; kilogram quantities can be evaluated for sea freight. The material ships as a non-hazardous solid, which keeps freight handling straightforward, and storage after arrival is −20 °C, protected from light and moisture.
Is there a minimum order quantity?
The practical minimum is the 100 mg research pack, which doubles as the evaluation sample for qualification programs. There is no separate MOQ policy above that — the six-tier pack ladder exists precisely so that quantities can scale from a single vial to 10 kg without renegotiating the supply relationship. Custom pack sizes outside the ladder are also possible on request.
Do you offer custom synthesis, custom purity or urolithin analogs?
Yes. Beyond the catalog grades we take on custom purity targets, custom pack formats, and custom synthesis of urolithin analogs and isotope-labeled variants for internal-standard work. Custom projects are scoped around the target specification, the analytical package required, and the intended scale; they are quoted individually after a short technical review of the request.
How does Urolithin A differ from Urolithin B when sourcing both?
They are distinct compounds, not interchangeable grades: Urolithin A (CAS 1143-70-0) has two hydroxyl groups (3,8-positions) and is the reference mitophagy tool compound, while Urolithin B (CAS 1139-83-9) has one (3-position) and is studied in NF-κB and TLR4-linked signaling contexts. Urolithin B is supplied in a single ≥99% grade, so direct per-gram price comparisons between the two only make sense once purity definition, pack size and delivery terms are aligned. The full comparison is in the Urolithin A vs B comparison article and the Urolithin B compound profile.
Is Urolithin A approved for human use?
No. The material NutraBiotechChem supplies is a research-grade chemical intended strictly for laboratory research, and it is not approved as a drug, food additive or dietary supplement ingredient by FDA, EMA or any other authority; it is not sold for human or veterinary use. All packs are labelled For Research Use Only, and general product, documentation and ordering questions are covered on the site FAQ page.

Why Source Urolithin A from NutraBiotechChem

What a repeat-purchase supply relationship looks like in practice.

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Batch-level transparency

Instrument data published before you buy — the HPLC, LC-MS and NMR package on this page is the actual release package for the current batch.

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Manufacturer-direct

Synthesis and purification under one roof, which is what makes both the ≥98% stock grade and made-to-order kilogram batches possible.

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Direct technical line

Quotations, COA copies and specification questions answered by the technical team — typically within one business day.

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Export experience

Research-chemical export documentation, non-hazardous freight handling and Incoterm flexibility for EU, US and Asia destinations.

Request Your Urolithin A Quotation

Send the pack size, grade and destination — a batch-data-backed quotation comes back within one business day. Samples start at the 100 mg research pack.

Request Quotation

Or reach the team directly via WhatsApp (+86 177 8639 4783) or the contact page.

For Research Use Only. Urolithin A (CAS 1143-70-0) supplied by NutraBiotechChem is a laboratory research chemical, not a drug, food additive or dietary supplement ingredient, and is not approved for human or veterinary use by FDA, EMA or any other regulatory authority. Statements on this page describe the research material and its documentation, and are not health or medical claims.

Explore the Urolithin A range: Product specifications page · Price & grade guide · All research chemicals

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