LY294002 CAS 154447-36-6 98% Purity PI3K Inhibitor Research Powder

LY294002 CAS 154447-36-6 98% Purity PI3K Inhibitor Research Powder

High purity LY294002 powder CAS 154447-36-6 with 98% HPLC purity. Classic selective PI3K/Akt pathway inhibitor for tumor, autophagy and inflammation research, factory bulk supply with full certificates.
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LY294002 CAS 154447-36-6 | PI3K Inhibitor (p110) | Cancer & Autophagy Research | Nutrabiotech Chem

LY294002 CAS 154447-36-6
Selective PI3K Inhibitor (p110) for Signaling Research

LY294002 (CAS 154447-36-6) is a selective, cell-permeable inhibitor of phosphatidylinositol 3-kinase (PI3K) that targets the p110 catalytic subunit (IC50 ~1.4 μM). By blocking PI3K-dependent generation of PIP3, it suppresses the PI3K–AKT–mTOR signaling axis — a foundational tool for cancer biology, apoptosis, autophagy and cell-proliferation studies. Formula C19H17NO3, MW 307.34. Research grade with COA.

LY294002PI3K Inhibitorp110AKT/mTORCancer ResearchAutophagyQuercetin DerivativeSignaling
Request a Quote Technical Specs
307.34
MW (C19H17NO3)
~1.4 μM
PI3K IC50
p110
Subunit target

Molecular Information

Name: LY294002
CAS: 154447-36-6
Formula: C19H17NO3
MW: 307.34 g/mol
Class: Morpholine chromone (PI3K inhibitor)
Core: 2-(4-Morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Target: PI3K p110 catalytic subunit
Activity: Blocks PIP3 / PI3K–AKT–mTOR
MP: ~165–170 °C (decomp., reported)
Appearance: Yellow to off-white powder
Synonyms: LY-294002; 2-morpholino-8-phenylchromone
Storage: -20°C, dry, protect from light
154447-36-6
🧪
CAS Number
307.34
⚖️
Molecular Weight
PI3K p110
🎯
Primary Target
≥98%
✅
Purity

Product Technical Specifications

Complete physicochemical properties and QC parameters for LY294002 (CAS 154447-36-6)

📋 Physicochemical Properties

  • Product NameLY294002
  • IUPAC Name2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
  • CAS Number154447-36-6
  • SynonymsLY-294002; 2-morpholino-8-phenylchromone
  • Molecular FormulaC19H17NO3
  • Molecular Weight307.34 g/mol
  • SourceSynthetic (research inhibitor)
  • AppearanceYellow to off-white powder
  • Water SolubilityPoor (prepare DMSO stock)
  • Organic SolubilityDMSO (~25 mg/mL); ethanol
  • Melting Point~165–170 °C (decomp. reported)
  • Compound ClassMorpholine-substituted chromone
  • HS Code2932.99

🔬 Quality Control & Handling

  • Purity (HPLC)≥98%
  • FormPowder (research grade)
  • Primary TargetPI3K p110 catalytic subunit
  • Assay ReadoutPIP3 / AKT / mTOR suppression
  • SelectivityClass I PI3K (reversible)
  • Storage Condition-20°C, sealed, dry, protect from light
  • Solution StabilityDMSO stock stable at -20°C
  • ShippingRoom temp / cold pack
  • QC DocumentationCOA / HPLC / NMR / MSDS
  • Pack Sizes1g / 5g / 10g / 100g / 1KG
  • Stock StatusIn Stock
  • Use StatementResearch use only; not for human/clinical use

Key Molecular Targets & Biology Nodes

LY294002 engages the PI3K signaling node and the downstream cascade that governs proliferation, survival and metabolism.

🧬 PI3K (p110) 🧬 PIP3 (PIP2→PIP3) 🧬 AKT / PKB 🧬 mTOR (C1/C2) 🧬 PDK1 🔥 Apoptosis ♻️ Autophagy 🧫 Cell Proliferation 🩸 Angiogenesis 🧬 IGF-1 / Insulin signaling

How LY294002 Works: Blocking the PI3K–AKT–mTOR Axis

LY294002 occupies the PI3K p110 active site, halting PIP3 production and shutting down the survival and growth signals downstream of receptor tyrosine kinases.

1

Inhibition of PI3K (p110)

LY294002 reversibly binds the PI3K p110 catalytic subunit (IC50 ~1.4 μM), preventing phosphorylation of PIP2 to PIP3 at the inner leaflet of the plasma membrane.

2

Suppression of AKT Recruitment

Without PIP3, AKT (and PDK1) fail to translocate and become activated. Phosphorylation of AKT, GSK-3β and downstream targets drops, blunting pro-survival signaling.

3

Loss of mTOR & Growth Output

Reduced AKT/mTOR activity lowers cap-dependent translation, cell-cycle progression and angiogenesis while sensitizing cells to apoptosis and inducing autophagy.

⚖️ LY294002 vs Comparators

  • LY294002 (this product)Reversible PI3K p110 inhibitor (~1.4 μM)
  • WortmanninIrreversible PI3K (sub-10 nM), broad
  • Class I selectivityTargets p110α/β/δ/γ
  • Selective EdgeStable, widely used cell-culture tool
  • Research NichePI3K/AKT/mTOR pathway dissection

♻️ Downstream Biological Consequences

  • LipidPIP3 ↓
  • KinaseAKT / mTOR ↓
  • Cell fateApoptosis ↑ / proliferation ↓
  • CatabolismAutophagy induced
  • VesselAngiogenesis suppressed
  • ModelsCancer / insulin-signaling studies

Research & Development Applications

LY294002 is a foundational small-molecule tool for dissecting PI3K-dependent signaling across oncology, metabolism and cell biology.

🧬

Cancer Biology

Probes PI3K–AKT–mTOR dependence, proliferation, invasion and chemo-/radio-resistance in tumor models.

Oncology
🔥

Apoptosis & Survival

Sensitizes cells to death ligands and cytotoxic agents by removing pro-survival AKT tone.

Apoptosis
♻️

Autophagy Studies

Induces autophagic flux via mTOR inhibition for flux and lysosome-readout assays.

Autophagy
🩸

Angiogenesis

Suppresses PI3K-driven endothelial migration and tube formation in vascular research.

Angiogenesis
🍬

Metabolic Signaling

Dissects insulin/IGF-1 and glucose-transport signaling in metabolic models.

Metabolism
🧪

Pathway Comparators

Paired with mTOR or MEK inhibitors to separate PI3K versus MAPK contributions.

Comparator

Key Literature & Landmark Publications

Landmark and representative work establishing LY294002 as the canonical PI3K (p110) inhibitor.

Vlahos C, et al. 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002) inhibits PI3K and blocks mitogenic signaling.
Landmark reference — J. Biol. Chem. 1994
Review. PI3K–AKT–mTOR pathway in cancer: roles of LY294002 and wortmannin.
Landmark reference
Preclinical studies. LY294002 synergizes with cytotoxic agents in PI3K-dependent tumors.
Landmark reference
Mechanistic work. AKT/PKB phosphorylation downstream of PI3K blocked by LY294002.
Landmark reference
Autophagy literature. PI3K inhibition by LY294002 induces autophagic flux.
Landmark reference

Available Sizes & Ordering

Research-grade LY294002 (CAS 154447-36-6) with full QC documentation; standard packs and bulk custom quantities supported.

TierPack SizeStock StatusSuitable ForLead Time
Standard1 gIn StockLab screening / assaysSame/next-day ship
Medium5 gIn StockExpanded studiesSame/next-day ship
Large10 gIn StockProject-scale useSame/next-day ship
Bulk100 gIn StockHigh-throughput / screeningQuote to confirm
Industrial1 KG+Made to orderProcess developmentBatch delivery

💡 Reference sizes shown above; for exact pricing and availability please contact us for a quote. Bulk orders qualify for tiered discounts.

Frequently Asked Questions (FAQ)

What is LY294002 and what pathway does it inhibit?
LY294002 (CAS 154447-36-6) is a selective, cell-permeable inhibitor of class I phosphatidylinositol 3-kinase (PI3K) that binds the p110 catalytic subunit (IC50 ~1.4 μM). It blocks PI3K-dependent production of PIP3, thereby suppressing the downstream PI3K–AKT–mTOR signaling axis. Formula C19H17NO3, MW 307.34.
How does LY294002 differ from wortmannin?
Both inhibit PI3K, but wortmannin is an irreversible, nanomolar (sub-10 nM) inhibitor with broader off-target kinase engagement, while LY294002 is a reversible, micromolar (~1.4 μM) inhibitor derived from the flavonoid quercetin and generally more stable in cell culture. LY294002 is the more commonly used small-molecule tool for routine PI3K blockade.
What research areas use LY294002?
LY294002 is used in cancer biology (proliferation, apoptosis, chemo-resistance), autophagy, angiogenesis, insulin/IGF-1 signaling, stem-cell and immunology studies, and as a comparator when dissecting PI3K–AKT–mTOR versus MAPK pathway contributions.
How soluble is LY294002 and how should it be prepared?
LY294002 is poorly soluble in water but readily soluble in DMSO (up to ~25 mg/mL, ~80 mM). Prepare a sterile-filtered DMSO stock, aliquot and store at -20°C protected from light. Avoid repeated freeze-thaw. It is for research use only, not for human or clinical use.
Is QC documentation provided?
Every batch ships with a Certificate of Analysis (COA) including HPLC purity, structural confirmation (NMR/HMBC) and identity data; MSDS/SDS and Certificates of Origin are available on request.

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Research-grade LY294002 (CAS 154447-36-6) — purity ≥98% HPLC, COA included
Selective PI3K (p110) inhibitor (IC50 ~1.4 μM) — request a quote today

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