KY19382 A3051 powder CAS 2226664-93-1 research compound

KY19382 CAS 2226664-93-1 Wnt/β-Catenin Activator & Dual CXXC5-Dvl / GSK3β Inhibitor

KY19382 (A3051, CAS 2226664-93-1) is a Wnt/β-catenin activator, dual CXXC5-DVL and GSK3β inhibitor for hair regrowth and wound healing research. KG stock available, COA provided, for research use only.

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KY19382 CAS 2226664-93-1 98% Purity Wnt Activator Powder - NutraBiotech

KY19382 CAS 2226664-93-1
Wnt/β-Catenin Activator & Dual CXXC5-Dvl / GSK3β Inhibitor

KY19382 (CAS 2226664-93-1) is a synthetic analogue of indirubin-3′-monoxime that activates Wnt/β-catenin signalling through two complementary nodes: it disrupts the protein-protein interaction between the negative feedback regulator CXXC5 and Dishevelled (IC50 = 19 nM) and directly inhibits glycogen synthase kinase 3β (IC50 = 10 nM). Published preclinical work covers hair regrowth and wound-induced hair follicle neogenesis, growth-plate driven longitudinal bone growth, chondrogenesis and high-fat-diet metabolic models. Formula C17H11Cl2N3O2, MW 360.19. Research grade, ≥98% HPLC, shipped with COA.

KY19382CXXC5-DvlGSK3βWnt/β-cateninHair Follicle NeogenesisDermal PapillaAlopecia ResearchGrowth Plate
Request a Quote Technical Specs
360.19
MW (C17H11Cl2N3O2)
≥98%
Purity (HPLC)
19 / 10 nM
CXXC5-Dvl / GSK3β IC50

Molecular Information

Name: KY19382
CAS: 2226664-93-1
Formula: C17H11Cl2N3O2
MW: 360.19 g/mol
Exact Mass: 359.0230
Class: Indirubin-3′-monoxime (I3O) analogue
Scaffold: 5,6-dichloro bis-indole oxime
Target 1: CXXC5-Dvl interaction (19 nM)
Target 2: GSK3β (10 nM)
Pathway: Wnt/β-catenin activation
Appearance: Brown to reddish-brown solid
Storage: -20 °C, dry, protect from light
2226664-93-1
🧪
CAS Number
360.19
⚖️
Molecular Weight
CXXC5-Dvl
🎯
Primary Target
≥98%
✅
Purity (HPLC)

Product Technical Specifications

Complete physicochemical identity and QC parameters for KY19382 (CAS 2226664-93-1)

📋 Physicochemical Identity

  • Product NameKY19382
  • IUPAC Name5,6-dichloro-3-[1,3-dihydro-3-(methoxyimino)-2H-indol-2-ylidene]-1,3-dihydro-2H-indol-2-one
  • CAS Number2226664-93-1
  • SynonymsKY 19382; KY-19382; I3O analogue; Wnt/β-catenin signalling activator
  • Molecular FormulaC17H11Cl2N3O2
  • Molecular Weight360.19 g/mol (exact mass 359.0230)
  • Elemental AnalysisC 56.69; H 3.08; Cl 19.68; N 11.67; O 8.88
  • Chemical ClassSynthetic bis-indole oxime (indirubin-3′-monoxime analogue)
  • SMILESCO\N=C1/C(/Nc2ccccc/12)=C1/C(=O)Nc2cc(Cl)c(Cl)cc12
  • InChIKeyPROTUMHDKVZVBW-CLXIBKRSSA-N
  • InChIKey (no stereo)PROTUMHDKVZVBW-UHFFFAOYSA-N
  • PubChem CID148959784
  • AppearanceBrown to reddish-brown solid powder
  • SolubilityDMSO ≈4–5 mg/mL (11.6–13.9 mM); warming/sonication advised
  • Aqueous SolubilityPractically insoluble in water
  • HS Code2934.99 (reference vendor listing; confirm per destination)

🔬 Quality Control & Handling

  • Purity (HPLC)≥98%
  • FormSolid powder
  • Primary TargetsCXXC5-Dvl interaction; GSK3β
  • Reported PotencyCXXC5-Dvl IC50 19 nM; GSK3β IC50 10 nM
  • Assay ReadoutsTOPFlash reporter; β-catenin; p-GSK3β (S9); PCNA; ALP
  • Cell ModelsATDC5; C28/I2; HEK293 reporter; human dermal papilla
  • Storage (powder)-20 °C up to 3 years; 4 °C up to 2 years
  • Storage (in solvent)-80 °C up to 6 months; -20 °C up to 1 month
  • ShippingAmbient, non-hazardous; cold chain on request
  • QC DocumentationCOA / HPLC / NMR / MS / MSDS
  • Pack Sizes100 mg / 1 g / 10 g / 100 g / 1 kg / 10 kg
  • Stock StatusIn Stock
  • Development StatusPreclinical (no marketing authorisation)
  • Use StatementResearch use only; not for human or veterinary use

Key Molecular Targets & Pathway Nodes

KY19382 acts on the CXXC5 negative-feedback arm and the GSK3β destruction complex, converging on β-catenin driven transcription.

🧬 CXXC5 🧬 Dishevelled (Dvl) 🧪 GSK3β 🔵 β-catenin 📈 TOPFlash reporter 🧴 Dermal papilla cells 🌱 Hair follicle neogenesis 🦴 Growth plate / chondrogenesis 🍃 Metabolic disease models 🧭 Wnt target genes (Axin2, WISP1)

How KY19382 Works: Releasing the Brake on Wnt/β-Catenin Signalling

Two complementary actions in one molecule — upstream block of the CXXC5-Dvl negative feedback loop and direct inhibition of GSK3β.

1

Block of the CXXC5–Dvl Interaction

CXXC5 is a negative-feedback regulator of the Wnt pathway that binds Dishevelled and dampens signal transduction. KY19382 disrupts this protein-protein interaction (IC50 = 19 nM), removing the brake at the receptor-proximal level.

2

Direct Inhibition of GSK3β

KY19382 inhibits glycogen synthase kinase 3β (IC50 = 10 nM), lowering destruction-complex activity and raising inhibitory phospho-GSK3β (Ser9) levels, an activity independent of the CXXC5 arm.

3

β-Catenin Stabilisation & Transcription

Stabilised β-catenin accumulates in cytosol and nucleus; TOPFlash reporter activity rises (0.001–10 μM, 18 h) together with Wnt target genes Axin2 and WISP1, and with ALP and PCNA in human dermal papilla cells.

⚖️ KY19382 vs Comparator Tools

  • KY19382 (this product)Dual CXXC5-Dvl + GSK3β block
  • PTD-DBMCXXC5-Dvl peptide only
  • Valproic acid / LiClGSK3β-level activators only
  • WAY-316606sFRP-1 inhibitor, Wnt-pathway hair tool
  • Finasteride / dutasteride5α-reductase, androgen axis
  • MinoxidilVasodilator, hair-growth comparator
  • Selective EdgeUpstream release + kinase block in one molecule
  • Research NicheFollicle neogenesis / bone / metabolic models

♻️ Downstream Biological Consequences

  • β-cateninStabilised, nuclear translocation up
  • TranscriptionAxin2, WISP1 mRNA up
  • Dermal papillaALP activity and PCNA up
  • Ex vivo follicleVibrissa and human hair shaft elongation
  • In vivo hairRegrowth and de novo follicle neogenesis
  • BoneGrowth-plate proliferation, tibia length up
  • CartilageChondrogenic marker induction (ATDC5, C28/I2)
  • MetabolicEvaluated in high-fat-diet models

Research & Formulation Applications

KY19382 is used as a Wnt-pathway tool compound across hair-follicle, skeletal, cartilage and metabolic research programmes.

🧴

Hair Loss & Alopecia Models

Human dermal papilla cells, ex vivo vibrissa and human hair follicle culture, depilation-induced hair cycling.

Alopecia
🌱

Wound-Induced Follicle Neogenesis

WIHN and hair patch assays in C57BL/6 mice; Fgf9 and keratin 17 as neogenesis markers.

Neogenesis
🧬

Follicle Stem Cell Activation

Keratin 15-positive bulge readouts with β-catenin, Ki67 and PCNA immunostaining.

Stem Cells
🦴

Bone Growth & Growth Plate

Growth-plate senescence and longitudinal bone growth; 0.1 mg/kg per day raised tibia length in adolescent mice.

Bone
🦠

Chondrogenesis & Cartilage

ATDC5 and C28/I2 chondrogenic differentiation markers at 0.01–0.1 μM.

Cartilage
🍃

Metabolic Disease Models

High-fat-diet induced models; the scaffold is the subject of patent WO2020079569 on metabolic disease.

Metabolic

Key Literature & Landmark Publications

Peer-reviewed work defining KY19382 pharmacology across hair-follicle, skeletal and metabolic models.

Ryu YC, Lee DH, Shim J, Park J, Kim YR, Choi S, et al. KY19382, a novel activator of Wnt/β-catenin signalling, promotes hair regrowth and hair follicle neogenesis. Br J Pharmacol. 2021;178(12):2533-2546.
DOI: 10.1111/bph.15438 · PMID: 33751552
Choi S, Kim HY, Cha PH, Lee C, Choi Y, Han G, et al. CXXC5 mediates growth plate senescence and is a target for enhancement of longitudinal bone growth. Life Sci Alliance. 2019;2(2):e201800254.
Bone growth reference
Ryu YC, Kim YR, Park J, et al. Wnt/β-catenin signaling activator restores hair regeneration suppressed by diabetes mellitus. BMB Rep. 2022;55(11):559-564.
Diabetes / hair reference
Ryu YC, et al. CXXC5 mediates DHT-induced androgenetic alopecia via PGD2. Cells. 2023;12(4):555.
DOI: 10.3390/cells12040555 · PMID: 36831222
Lee SH, Seo SH, Lee DH, Pi LQ, Lee WS, Choi KY. Targeting of CXXC5 by a competing peptide stimulates hair regrowth and wound-induced hair neogenesis. J Invest Dermatol. 2017;137(11):2260-2269.
DOI: 10.1016/j.jid.2017.04.038 · PTD-DBM reference
Choi KY, et al. Compositions and methods for suppressing and/or treating metabolic diseases and/or a clinical condition thereof. WO2020079569.
Patent reference

Supply, IP & Compliance Notes

Status of the molecule and documentation that accompanies each shipment.

⚠️ Regulatory Position

  • Development StagePreclinical
  • Marketing AuthorisationNone in any jurisdiction
  • Intended UseLaboratory research only
  • Human / Veterinary UseNot permitted
  • Originator OrganisationsCK Regeon Inc.; Yonsei University
  • Active Indication (pipeline)Alopecia

📦 Documentation & Logistics

  • Certificate of AnalysisBatch-specific, HPLC purity
  • Structural ConfirmationNMR and MS data
  • Safety DocumentationMSDS / SDS on request
  • Origin DocumentsCertificate of Origin available
  • Transport ClassificationNon-hazardous chemical, ambient
  • IP ConsiderationComposition/method patents filed by originators

Available Sizes & Ordering

Research-grade KY19382 (CAS 2226664-93-1) with full QC documentation; supplied from 100 mg research packs through to 10 kg production batches.

TierPack SizeStock StatusSuitable ForLead Time
Research100 mgIn StockTOPFlash, β-catenin and DP-cell assaysSame/next-day ship
Standard1 gIn StockExtended in vitro and ex vivo programmesSame/next-day ship
Scale-up10 gIn StockIn vivo dose-ranging and formulation screening3–5 business days
Pilot100 gMade to orderProcess development and pilot batchesQuote to confirm
Commercial1 kgMade to orderContract supply and validation runsBatch delivery
Industrial10 kgMade to orderLarge-scale production schedulingReserved batch slot

Reference sizes shown above; for exact pricing and availability please contact us for a quote. Bulk orders qualify for tiered discounts.

Frequently Asked Questions (FAQ)

What is KY19382 and what is it researched for?
KY19382 (CAS 2226664-93-1) is a synthetic analogue of indirubin-3′-monoxime and an activator of Wnt/β-catenin signalling. It is studied in hair regrowth and wound-induced hair follicle neogenesis, growth-plate driven longitudinal bone growth, chondrogenesis, and high-fat-diet metabolic disease models. Molecular formula C17H11Cl2N3O2, molecular weight 360.19.
How does KY19382 activate Wnt/β-catenin signalling?
KY19382 acts at two nodes. It disrupts the interaction between the negative feedback regulator CXXC5 and Dishevelled, releasing the brake at the receptor-proximal level, and it directly inhibits GSK3β, which lowers destruction-complex activity. Stabilised β-catenin then accumulates and drives transcription of Wnt target genes including Axin2 and WISP1.
What are the reported IC50 values for KY19382?
Published values are IC50 = 19 nM for inhibition of the CXXC5-Dvl protein-protein interaction and IC50 = 10 nM for inhibition of GSK3β. Both figures come from the characterisation work behind the Life Science Alliance 2019 and British Journal of Pharmacology 2021 studies.
Is KY19382 the same compound as PTD-DBM?
No. PTD-DBM is a peptide that interferes with the CXXC5-Dvl interaction only, whereas KY19382 is a small molecule that combines CXXC5-Dvl interference with direct GSK3β inhibition. In a dihydrotestosterone model, KY19382 fully restored β-catenin signalling where single-node interventions gave partial results.
Which experimental models has KY19382 been used in?
Published models include human dermal papilla cells, ex vivo mouse vibrissa and human hair follicle culture, depilation-induced hair regrowth, wound-induced hair follicle neogenesis and hair patch assays in C57BL/6 mice, ATDC5 and C28/I2 chondrogenic cells, HEK293 TOPFlash reporter cells, and adolescent mice for growth-plate and tibia-length endpoints.
How soluble is KY19382 and how are stock solutions prepared?
KY19382 is soluble in DMSO at roughly 4 to 5 mg/mL (11.6 to 13.9 mM) and practically insoluble in water. Reported protocols use warming to about 80 °C with sonication and freshly opened DMSO, since hygroscopic DMSO lowers apparent solubility. Aliquoted stocks are stored at -80 °C for up to 6 months or -20 °C for up to 1 month, avoiding repeated freeze-thaw cycles.
How should KY19382 be stored and shipped?
Solid KY19382 is stored at -20 °C protected from light, with a reported shelf life of up to 3 years at -20 °C and 2 years at 4 °C. It ships at ambient temperature as a non-hazardous chemical and is stable for the ordinary transit and customs period; cold-chain shipment is available on request.
What QC documentation is provided with each batch?
Every batch ships with a batch-specific Certificate of Analysis reporting HPLC purity, together with NMR and MS structural confirmation. MSDS/SDS and Certificates of Origin are available on request.
Which pack sizes are available and is bulk supply supported?
KY19382 is offered in six standard sizes: 100 mg, 1 g, 10 g, 100 g, 1 kg and 10 kg. The 100 mg, 1 g and 10 g sizes are held in stock, while 100 g, 1 kg and 10 kg are made to order against a quotation. Bulk quantities qualify for tiered discounts, and lead time is quoted per batch.
Is KY19382 approved for human or veterinary use?
No. KY19382 is at the preclinical stage with no marketing authorisation in any jurisdiction and is supplied strictly for laboratory research use. It is not intended for diagnostic or therapeutic procedures and not for human or veterinary administration.
Which related hair-loss research compounds are available?
Related tool compounds for hair-follicle research include WAY-316606, an sFRP-1 inhibitor acting on the same Wnt pathway, plus anti-androgen and comparator actives listed in the hair loss solution ingredients category.

Need KY19382 for Your Research?

Research-grade KY19382 (CAS 2226664-93-1) — purity ≥98% HPLC, COA included
CXXC5-Dvl IC50 19 nM / GSK3β IC50 10 nM — request a quote today

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KY19382 (CAS 2226664-93-1) is supplied for laboratory research use only. It is not a drug, not a dietary ingredient, and is not for human or veterinary use, diagnosis or treatment. Development status is preclinical, and composition and method patents covering the scaffold have been filed by the originating organisations; patent status should be confirmed for the intended territory before any commercial activity.

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