Nutrabiotech WAY 316606 98% 1G Powder, high purity WAY-316606 research powder, selective sFRP-1 inhibitor, Wnt/β-catenin signaling activator, osteogenesis and hair regeneration research raw material

WAY 316606 CAS 915759-45-4 98% High-Purity Powder SFRP-1 Inhibitor Wnt/β-Catenin Activator Hair Follicle Growth Bone Formation

Premium 98% WAY 316606 crystalline powder CAS 915759-45-4, selective sFRP-1 inhibitor and Wnt/β-catenin signaling activator. Ideal for hair regrowth, anti-hair loss and bone formation pharmacological research.
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WAY-316606 (CAS 915759-45-4) SFRP1 Antagonist | Wnt/β-Catenin Activator for Hair-Growth & Bone Research Compound

WAY-316606 CAS 915759-45-4
SFRP1 Antagonist & Canonical Wnt/β-Catenin Activator

WAY-316606 (CAS 915759-45-4) is a specific antagonist of secreted frizzled-related protein 1 (SFRP1) that activates canonical Wnt/β-catenin signaling in the human hair bulb (EC50 ~0.65 µM). Molecular formula C18H19F3N2O4S2, MW 448.48. Research-grade with COA.

SFRP1 Antagonist WAY-316606 Wnt beta-catenin Hair Growth Bone Formation Androgenetic Alopecia Frizzled
Request a Quote Technical Specs
448.48
MW (C18H19F3N2O4S2)
~0.65 µM
Wnt3 EC50
SFRP1
Specific antagonist

Molecular Information

Name: WAY-316606
CAS: 915759-45-4
Formula: C18H19F3N2O4S2
MW: 448.48 g/mol
Class: Bis-sulfonamide SFRP1 antagonist
Core: 5-(benzenesulfonyl)-N-piperidin-4-yl-2-(trifluoromethyl)benzene-1-sulfonamide
Target: SFRP1 (Wnt antagonist)
EC50: ~0.65 µM (Wnt reporter)
Appearance: White to off-white powder
Synonyms: SFRP1 antagonist
Storage: -20 C
🧪
CAS Number
915759-45-4
⚖️
Molecular Weight
448.48
🎯
Primary Activity
SFRP1 Antagonist
✅
Purity
≥98%

Product Technical Specifications

Complete physicochemical properties and QC parameters for WAY-316606 (CAS 915759-45-4)

📋 Physicochemical Properties

  • Product NameWAY-316606
  • IUPAC Core5-(benzenesulfonyl)-N-(piperidin-4-yl)-2-(trifluoromethyl)benzene-1-sulfonamide
  • CAS Number915759-45-4
  • SynonymsSFRP1 antagonist; WAY 316606
  • Molecular FormulaC18H19F3N2O4S2
  • Molecular Weight448.48 g/mol
  • SourceSynthetic small molecule (Wayne State / Wnt program)
  • AppearanceWhite to off-white powder
  • Melting PointNot reported
  • Water SolubilityInsoluble
  • Organic SolubilityDMSO ~90 mg/mL; ethanol
  • Compound ClassBis-sulfonamide / SFRP1 antagonist
  • HS Code2934.99

🔬 Quality Control & Handling

  • Purity (HPLC)≥98%
  • FormSolid powder
  • Primary TargetSFRP1 (secreted frizzled-related protein 1)
  • Pathway ReadoutCanonical Wnt/β-catenin activation; β-catenin ↑; LEF/TCF reporter
  • SelectivitySpecific SFRP1 antagonist; releases Wnt for frizzled/LRP
  • Storage Condition-20 C, sealed, protect from light
  • Solution StabilityAliquot DMSO stocks; avoid freeze-thaw
  • ShippingBlue ice / cold chain recommended
  • QC DocumentationCOA / HPLC / NMR / MS / MSDS
  • Pack Sizes1g / 5g / 10g / 100g / 1KG
  • Stock StatusIn Stock
  • Use StatementResearch use only; not for human/clinical use

Key Molecular Targets & Pathway Nodes

WAY-316606's node is SFRP1, an endogenous Wnt antagonist; by blocking SFRP1 it releases Wnt ligands to engage the frizzled/LRP receptor complex and turn on canonical Wnt signaling.

🧬 SFRP1 (secreted frizzled-related protein 1) 🧬 Wnt Ligands 🔗 Frizzled / LRP5/6 Receptor 🔗 β-Catenin 🧱 DVL / Destruction Complex 🧱 LEF/TCF Transcription 🧱 Hair Follicle (bulge / DP) 🦴 Osteoblast / Bone 🧱 Hair Shaft Keratins 🧪 Anagen Promotion 🔬 Catagen Inhibition ⚡ Canonical Wnt Pathway

How WAY-316606 Works: Freeing Wnt by Blocking its Inhibitor SFRP1

WAY-316606 binds SFRP1 and out-competes Wnt, releasing Wnt to activate frizzled/LRP and canonical β-catenin signaling

1

SFRP1 Blockade Releases Wnt

SFRP1 is a secreted Wnt antagonist that mops up Wnt ligands. WAY-316606 specifically binds SFRP1 and out-competes Wnt for SFRP1, freeing Wnt proteins to engage the frizzled/LRP receptor complex.

2

Canonical Wnt/β-Catenin Activation

With Wnt free to signal, the destruction complex is inhibited, β-catenin stabilizes and translocates to the nucleus to drive LEF/TCF target-gene transcription. In the human hair bulb this boosts β-catenin activity in pre-cortex keratinocytes and dermal-papilla fibroblasts (EC50 ~0.65 µM).

3

Hair-Shaft Production & Anagen Support

Ex vivo, WAY-316606 enhances hair-shaft production and hair-shaft keratin expression while inhibiting spontaneous follicle regression (catagen). The same Wnt activation also promotes anabolic bone formation, linking it to osteoporosis research.

⚖️ WAY-316606 vs Wnt Agonists / Antagonists

  • WAY-316606 (this product)Specific SFRP1 antagonist; releases endogenous Wnt
  • Wnt3a (ligand)Direct Wnt ligand addition
  • Porcupine inhibitorsOpposite — block Wnt secretion
  • Shared PathwayCanonical Wnt/β-catenin
  • Key DistinctionTargets the endogenous Wnt inhibitor SFRP1
  • Tissue LinkHair bulb & bone anabolism

♻️ Downstream Biological Consequences

  • AntagonistSFRP1 bound / inactivated
  • LigandWnt freed for frizzled/LRP
  • Signalβ-catenin stabilized & nuclear
  • TranscriptionLEF/TCF target genes ↑
  • HairHair-shaft ↑; catagen inhibited
  • BoneAnabolic bone formation ↑

Research Applications & Models

A Wnt-pathway tool bridging hair-regrowth and bone-anabolism research via SFRP1 antagonism.

💇

Hair-Growth (Androgenetic Alopecia) Models

The flagship use: WAY-316606 enhances hair-shaft production and inhibits catagen in ex vivo human scalp explants via Wnt/β-catenin — a candidate hair-regrowth strategy.

Wnt / Hair bulb
🦴

Bone Formation & Osteoporosis

Originally identified as a bone-anabolic SFRP1 antagonist; studied for osteoblast activity and skeletal regeneration.

Wnt / Bone
🧪

Canonical Wnt/β-Catenin Research

A fiducial SFRP1 antagonist for LEF/TCF reporter, β-catenin and frizzled/LRP assays.

Wnt pathway
🔬

Hair-Follicle Biology

Used to probe anagen/catagen regulation and dermal-papilla – keratinocyte crosstalk.

Follicle cycle
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Regenerative Signaling

Studied in stem-cell and tissue-regeneration contexts where Wnt tone matters.

Regeneration
⚖️

Pathway Modulator Panel

Benchmarked against Wnt ligands and porcupine inhibitors to separate SFRP1 blockade from ligand addition.

Wnt panel

Key Literature & Landmark Publications

The discovery and hair/bone biology of WAY-316606 as an SFRP1 antagonist.

Ex vivo human scalp study: WAY-316606 enhances hair-shaft production and inhibits catagen via canonical Wnt/β-catenin signaling (PMC5940179, 2018).
Landmark reference
Guidetopharmacology: WAY-316606 identified as specific SFRP1 antagonist activating Wnt/β-catenin in the hair bulb.
Landmark reference
Selleck datasheet: WAY-316606 EC50 0.65 µM (U2OS WNT3 reporter); >99% purity.
Landmark reference
Original SFRP1-antagonist identification & bone-anabolism program (Wnt pathway).
Landmark reference
J Bone Miner Res. 2021: SFRP1 inhibitor WAY-316606 attenuates osteoclastogenesis via Wnt signaling.
doi: 10.1002/jbmr.4435

Available Sizes & Ordering

Research-grade WAY-316606 (CAS 915759-45-4) with full QC documentation; standard packs and bulk custom quantities supported.

TierPack SizeStock StatusSuitable ForLead Time
Standard1gIn StockSFRP1 / Wnt & hair-bulb assaysCold chain
Medium5gIn StockHair, bone & Wnt modelsCold chain
Large10gIn StockLong-term in vivo & formulation studiesCold chain
Bulk100gIn StockFormulation & process developmentQuote to confirm
Industrial1KGMade to orderPilot/production scale, CMO supplyBatch delivery

💡 Reference sizes shown above; for exact pricing and availability please contact us for a quote. Bulk orders qualify for tiered discounts.

Frequently Asked Questions (FAQ)

What is WAY-316606 and what does it do?
WAY-316606 (CAS 915759-45-4) is a specific antagonist of SFRP1 (secreted frizzled-related protein 1), an endogenous Wnt inhibitor. By binding SFRP1 it releases Wnt ligands to activate canonical Wnt/β-catenin signaling — boosting hair-shaft production and bone formation. Formula C18H19F3N2O4S2, MW 448.48; Wnt3-reporter EC50 ~0.65 µM.
How is WAY-316606 different from adding Wnt ligand?
Adding Wnt3a supplies ligand directly; WAY-316606 instead blocks the body's own Wnt inhibitor (SFRP1), freeing endogenous Wnt to signal. This acts upstream of the receptor and is tissue-tunable by local SFRP1 levels — distinct from porcupine inhibitors that block Wnt secretion.
What is the mechanism in hair growth?
In the human hair bulb, SFRP1 normally dampens Wnt. WAY-316606 binds SFRP1, allowing Wnt to engage frizzled/LRP, stabilize β-catenin and drive LEF/TCF transcription. Ex vivo this increases hair-shaft keratin expression and production while inhibiting spontaneous follicle regression (catagen) — the basis of its hair-regrowth research.
What are the key experimental readouts?
Readouts include LEF/TCF luciferase reporter activity (EC50 ~0.65 µM), nuclear β-catenin, hair-shaft keratin expression, and ex vivo human scalp explant hair counts / catagen scoring.
How soluble is WAY-316606 and how prepared?
WAY-316606 is highly soluble in DMSO (~90 mg/mL) and ethanol, but water-insoluble. For cell assays dilute DMSO stock into medium (final DMSO <=0.1% v/v); for in vivo use CMC-Na suspensions or validated vehicles. Store the solid at -20 C, desiccated and protected from light; aliquot stocks.
Is QC documentation provided?
Every batch ships with a Certificate of Analysis (COA) including HPLC purity, NMR structural confirmation and MS data. MSDS/SDS and Certificates of Origin are available on request. Sample evaluation is available for qualified institutions — please contact us.

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Research-grade WAY-316606 (CAS 915759-45-4) — purity ≥98% HPLC, COA included
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